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主動式染色機




 


Rapid Immunostaining
with Unparalleled Uniformity


Fast, Easy, and Efficient

Fast: Label large, intact samples such as rodent organs an order of magnitude faster than with passive labeling (≤ 24 hours vs. weeks to months).

Easy: Turnkey operation – simply load the buffers and your sample & probes. Perform 2 distinct labeling experiments simultaneously thanks to SmartLabel’s dual chamber design. 

Efficient: Uses a small amount of antibody (as little as ~3 µg) to label tissues the size of a whole mouse brain.

Reliable: Prevents tissue damage with Stochastic Electrotransport technology (Kim, PNAS, 2015). Our nanoporous membranes (patent-pending) eliminate tissue contamination and probe loss.

Complete and Uniform Labeling

Achieves unparalleled uniformity of signal intensity from surface to core using LifeCanvas’s advanced eFLASH (Yun, bioRxiv, 2019) protocol.
Combines rapid, Stochastic Electrotransport-mediated (Kim, PNAS, 2015) infiltration of probes into tissue with the SWITCH technique (Murray, Cell, 2015) of controlling probe binding kinetics.

Cost Savings by Avoiding Target Saturation

Prevents antibody depletion caused by excessive binding to superficial tissue sites and ensures the sample’s center is well-labeled.

 

Compatible with Many Cleared-Tissue Techniques








Immunolabeling at its fastest

SmartLabel is the world’s only active immunolabeling system, capable of staining large tissue samples in 24 hours or less. Each SmartLabel buffer kit contains enough labeling buffer for 5 staining experiments. 

Immunolabel a whole mouse brain (or sample of equivalent size) in ≤ 24 hours
Achieve uniform labeling from surface to core
Simple, plug-and-play buffer chemistry design
Buffers can be safely disposed of down the drain

Download the protocol below to learn more about the entire LifeCanvas tissue processing system, including active immunolabeling with SmartLabel.


 
 

Anti-GFAP Staining in CA1 Hippocampus of 2-mm Thick Section of Mouse Brain

Volume rendering of anti-GFAP staining in a 2-mm thick section of mouse brain, with signal in CA1 hippocampus most prominent. Labeling achieved in 2 days using antibody electrophoresis performed by SmartLabel. Cleared with SmartClear II Pro (<1 day), refractive index-matched with EasyIndex, and light-sheet imaged with SmartSPIM (<4 minutes acquisition time with 2-µm z-step and 1.8 µm/pixel XY; 2×2 tile using a 3.6x, 0.2 NA objective). See more videos in the video gallery!



 
 

SmartLabel: Rapid Immunostaining with Unparalleled Uniformity

SmartLabel, the world’s first active labeling device, combines SWITCH and stochastic electrotransport technologies to quickly and uniformly label whole tissue samples in a fraction of the time needed for passive labeling. Stain an entire mouse brain or rat hemisphere in 24 hours or less and avoid the time-intensive, destructive sectioning and tedious image reconstruction of traditional histology.